Confocal Systems
Leica TSC SP8 with lightning
- Type: Confocal laser scanning microscope
- Mode: Upright fixed stage, DM 6000 CFS, transmitted light - brightfield detector, fluorescence filters, DAPI (Emission: BP470/40); L5 (Emission: BP527/30); Rhodamine (Emission: BP585/40), motorized stage, stitching
- Detectors: (1xPMT, 3xHyD)
- Laser lines: 405nm, 488nm, 552nm, 638nm
- Deconvolution: HyVolution2
- Objectives:
- PL APO 10x/0.40
- PL APO 20x/0.75 IMM, CorrR (Water, Glycerin, Oil)
- FLUOTAR L 25x/0.95 W (Water Immersion)
- HC PLAPO 40x/1.30 Oil CS2
- PL APO 63x/1.30 Glyc. CorrR (Glycerin Immersion)
- PL APO 63/1.2 W Corr. (Water Immersion)
- Notch Filter (Excitation): 445, 488, 514, 448/514, 488/552/638, 594, 445/594
- Software: LAS X, Lightning
Leica TSC SP8 with FLIM
- Type: Confocal laser scanning microscope with FLIM
- Mode: Upright fixed stage, DM 6000 CFS
- Transmitted light – brightfield detector
- Laser lines: 405nm, 440nm, 470nm, 594nm, 633nm, and Aragon visible (488nm, 496nm, 514nm)
- Detectors: 3x Power HyD S and 2x T-PMT
- Objectives:
- HCPL FLUOTAR 10x N.A. 0.30 dry
- HC PL APO 20x N.A. 0.75 IMM CORR CS2 Water, Glycerin, Oil
- HC PL APO 63x N.A. 1.20 water
- HC APO UVis CS2 63X N.A. 0.90 W
- Add-ons: FLIM (Picoquant), motorized stage and stage navigator
- Software: LAS X
Widefield Systems
Leica THUNDER
- Type: THUNDER 4D inverse Imager, a Widefield Fluorescence Microscope with reconstructive deconvolution
- Objectives:
- HC PL FLUOTAR 5x N.A. 0.15
- HC PL FLUOTAR 10x N.A. 0.32
- HC FLUOTAR L 25x N.A. 0.95 W VISIR with cover glass
- HC PL APO 63x N.A. 1.20 W CORR CS2 IC-Prism D1
- HC PL APO 63x N.A. 1.40-0.60 OIL
- Add-ons: Two cameras: monochrome and color, sample navigator, Motorized XY Stage
- Software: Leica LAS X and Aivia
- Best used for: z-stack, time-lapse, tile-scan, multipoint, reconstructive deconvolution
Nikon SMZ18 Stereomicroscope
- Type: Stereo microscope SMZ18 with plan-apochromatic "Zoom Optics"
- Zoom factor: 18:1
- Zoom range: 0.75 - 13.5x
- Integrated double iris diaphragm in the zoom body
- Total magnification range when using 10x eyepieces: 3.75-270x (depending on objective selection)
- Motorized focusing unit
- Plan Apo 0.5x N.A. 0.075
- Working distance 71mm
- GFP-B Filter Cube: EX: 480/40 DM: 510, BA: 535/50
- RFP Filter Cube: EX: 545/30, DM: 570, BA: 620/60
- pHluorin2 Filter Cube: 492 SP Filter (excitation 395/475) 488 lp (dichroic) 525/50 (emission) filter cube
- Motorized microscope stage
Olympus BX61 Fluorescence Microscope (in room 1.602)
Binocular (Fluorescence): Zeiss Stereo Discovery V8 (DAPI/GFP/RhDx)
Zeiss Axiovert 40 CFL - Inverted Light Microscope/Fluorescence (DAPI, GFP, Ph1/2)
Zeiss ApoTome (Fluorescence) decentralized at AG Scholz