Confocal Systems
Leica TSC SP8 (Lightning)
- Type: Confocal laser scanning microscope
- Mode: upright fixed stage, DM 6000 CFS,
- transmitted light brightfield-detector, fluorescence filter, DAPI (emission: BP470/40); L5 (emission: BP527/30); rhodamine (emission: BP585/40), motorized stage, stitching
- Detectors: (1xPMT, 3xHyD)
- laser lines: 405 nm, 488 nm, 552 nm, 638 nm
- deconvolution: HyVolution2
- Objektives:
- PL APO 10x/0,40
- PL APO 20x/0,75 IMM, CorrR (glycerin, oil)
- FLUOTAR L 25x/0,95 W (water immersion)
- HC PLAPO 40x/1,30 oil CS2
- PL APO 63x/1,30 Glyc. CorrR (glycerin immersion)
- PL APO 63/1,2 W Corr. (water immersion)
- Notch Filter (excitation): 445, 488, 514, 448/514, 488/552/638, 594, 445/594
- Software: LAS X, Lightning
Leica TSC SP8 (FLIM)
- Type: Confocal laser scanning microscope
- Mode: upright fixed stage, DM 6000 CFS
- transmitted light brightfield-detector
- laser lines: 405 nm, 440 nm, 470 nm, 594 nm, 633 nm, and Aragon visible (488 nm, 496 nm, 514 nm)
- detectors: 3x Power HyD S und 2x T-PMT
- Objektives:
- HCPL FLUOTAR 10x N.A. 0,30 air
- HC PL APO 20x N.A. 0,75 IMM CORR CS2 water, glycerin, oil
- HC PL APO 63x N.A. 1,20 water
- HC APO UVis CS2 63X N.A. 0,90 water
- add-ons: FLIM (Picoquant), motorized stage and stage navigator
- Software: LAS X
Leica STELLARIS DIVE F TELLARIS DIVE FALCON
- Type: Multiphoton laser scanning microscope (Leica STELLARIS 8 DIVE/FALCON)
- Mode: Inverted microscope (DMi8), multiphoton imaging (DIVE), transmitted light (brightfield), motorized stage, stitching, live-cell compatible
- Detectors: Non-descanned spectral detectors (4Tune, HyD/PMT combinations), plus confocal spectral detectors (HyD S, HyD X, HyD R)
- Laser lines: Tunable IR femtosecond laser (Chameleon Vision II, 680–1080 nm), White Light Laser (440–790 nm), 405 nm diode
- Advanced imaging: Multiphoton imaging, FLIM (FALCON), TauSense (lifetime-based imaging), spectral imaging, FRAP, FRET, deep tissue imaging
- Scanning modes: Galvo and resonant scanning, ROI scanning, high-speed imaging
- Objectives:
- HC PL APO 10x/0.40 CS2
- HC PL APO 20x/0.75 IMM CORR (water, glycerol, oil)
- HC FLUOTAR L 25x/0.95 W VISIR
- HC PL IRAPO 40x/1.10 W motCORR (MP optimized)
- HC PL APO 40x/1.10 W CORR CS2
- HC PL APO 63x/1.20 W CORR CS2
- HC PL APO 63x/1.40 OIL CS2
- Detection range: ~380–850 nm (confocal and non-descanned detection)
- Multiphoton module: DIVE with flexible beam routing and tunable spectral detection
- Software: LAS X (incl. LIGHTNING, TauSense, Navigator, Dye Finder, FLIM analysis)
Widefield Systems
Leica THUNDER
- Type: THUNDER 4D inverse Imager, a Widefield Fluorescence Microscope with reconstructive deconvolution
- Objectives:
- HC PL FLUOTAR 5x N.A. 0.15
- HC PL FLUOTAR 10x N.A. 0.32
- HC FLUOTAR L 25x N.A. 0.95 W VISIR with cover glass
- HC PL APO 63x N.A. 1.20 W CORR CS2 IC-Prism D1
- HC PL APO 63x N.A. 1.40-0.60 OIL
- Add-ons: Two cameras: monochrome and color, sample navigator, Motorized XY Stage
- Software: Leica LAS X and Aivia
- Best used for: z-stack, time-lapse, tile-scan, multipoint, reconstructive deconvolution
Nikon SMZ18 Stereomicroscope
- Type: Stereo microscope SMZ18 with plan-apochromatic "Zoom Optics"
- Zoom factor: 18:1
- Zoom range: 0.75 - 13.5x
- Integrated double iris diaphragm in the zoom body
- Total magnification range when using 10x eyepieces: 3.75-270x (depending on objective selection)
- Motorized focusing unit
- Plan Apo 0.5x N.A. 0.075
- Working distance 71mm
- GFP-B Filter Cube: EX: 480/40 DM: 510, BA: 535/50
- RFP Filter Cube: EX: 545/30, DM: 570, BA: 620/60
- pHluorin2 Filter Cube: 492 SP Filter (excitation 395/475) 488 lp (dichroic) 525/50 (emission) filter cube
- Motorized microscope stage
Olympus BX61 Fluorescence Microscope
DAPI, FITC, TRIC, DIC
2x Air, 10x Air, 20x Air, 40x Air 60x oil, all APO
(in room 1.209)
Stereo Dissection Microscope (Fluorescence): Zeiss Stereo Discovery V8
DAPI/GFP/RhDx
Zeiss Axiovert 40 CFL - Inverted Light Microscope/Fluorescence
DAPI, GFP, Ph1/2
Zeiss ApoTome (Fluorescence)
FS38HE, FS43 HE, FS 46HE, FS49, FS50, FS64HE, DIC, Pol
Apo 5x Air, Apo 10x Air, Apo 20x Air, Apo 25x MIM, Apo 63x Oil
AxioCam ICc1
(in room 2.450)